Plasmid_Backbone

Part:BBa_K1480018

Designed by: Roberto Alejandro Valderrama Alfaro   Group: iGEM14_UANL_Mty-Mexico   (2014-10-17)


pSB3C5 + AF and BF TALEN (K1480001) Target sites

Backbone vector of pSB3C5 that contains two modifications with a TALEN target site after and before the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains two TALEN target sites (AF+BF)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2812
    Illegal XbaI site found at 2827
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2812
    Illegal NheI site found at 1450
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2818
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2812
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2812
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2827
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal AgeI site found at 1041
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 3613
    Illegal AgeI site found at 3725
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 288


[edit]
Categories
Parameters
None